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  • Ký hiệu PL/XG: 660.6
    Nhan đề: Cloning human Benchwarmer gene (BNCH) harboring E164K in vector pcDNA3.1 by site-directed mutagenesis method /
DDC 660.6
Tác giả CN Nguyen, Hoang Danh
Nhan đề Cloning human Benchwarmer gene (BNCH) harboring E164K in vector pcDNA3.1 by site-directed mutagenesis method / Nguyen Hoang Danh, Vu Minh Thiet
Thông tin xuất bản Ho Chi Minh city : Nguyen Tat Thanh University, 2020
Mô tả vật lý 6 p.
Tóm tắt Benchwarmer (BNCH) gene encodes an orphan transmembrane transporter belonging to the Major Facilitator Superfamily (MFS), facilitating the transport of ions, amino acids, simple sugars and recently lysolipids. The loss of BNCH function caused lethality in several animal models with neurodegeneration and senescence. At the cellular level, dysregulation of BNCH leads to adverse phenotypes of lysosome and also autophagy (i.e. dyshomeostasis, accumulation of carbohydrates and sphingolipids, and enlarged lysosome). However, the molecular function and ligand of BNCH protein remain to be unrevealed. This study aims to create a radical substitution change in human BNCH coding gene to knock out the protein functions. More specifically, lysine (K) was used to replace the glutamic acid residue 164 (E164K) which is conserved in many animals (fly, zebrafish, mouse and human) and this E164K mutation recapitulated BNCH mutant phenotype. In conclusion, BNCH harboring E164K (BNCH*) was successfully produced by site-directed mutagenesis and cloned into pcDNA.3.1 vector. The construct was transformed into E. coli OmniMAX and that provides a valuable cell assay to search for the molecular ligand of BNCH.
Từ khóa tự do Cloning
Từ khóa tự do Benchwarmer
Từ khóa tự do Đột Biến
Từ khóa tự do Lysosomal membrane protein
Từ khóa tự do Plasmid pcDNA3.1
Nguồn trích Tạp chí Khoa học và Công nghệ - Đại học Nguyễn Tất Thành 2020tr. 06-11 Số: 12
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008081223s2020 vm| vie
0091 0
039|y20210607221801|zngantk
082 |a660.6
100 |aNguyen, Hoang Danh
245 |aCloning human Benchwarmer gene (BNCH) harboring E164K in vector pcDNA3.1 by site-directed mutagenesis method / |cNguyen Hoang Danh, Vu Minh Thiet
260 |aHo Chi Minh city : |bNguyen Tat Thanh University, |c2020
300 |a6 p.
520 |aBenchwarmer (BNCH) gene encodes an orphan transmembrane transporter belonging to the Major Facilitator Superfamily (MFS), facilitating the transport of ions, amino acids, simple sugars and recently lysolipids. The loss of BNCH function caused lethality in several animal models with neurodegeneration and senescence. At the cellular level, dysregulation of BNCH leads to adverse phenotypes of lysosome and also autophagy (i.e. dyshomeostasis, accumulation of carbohydrates and sphingolipids, and enlarged lysosome). However, the molecular function and ligand of BNCH protein remain to be unrevealed. This study aims to create a radical substitution change in human BNCH coding gene to knock out the protein functions. More specifically, lysine (K) was used to replace the glutamic acid residue 164 (E164K) which is conserved in many animals (fly, zebrafish, mouse and human) and this E164K mutation recapitulated BNCH mutant phenotype. In conclusion, BNCH harboring E164K (BNCH*) was successfully produced by site-directed mutagenesis and cloned into pcDNA.3.1 vector. The construct was transformed into E. coli OmniMAX and that provides a valuable cell assay to search for the molecular ligand of BNCH.
653 |aCloning
653 |aBenchwarmer
653 |aĐột Biến
653 |aLysosomal membrane protein
653 |aPlasmid pcDNA3.1
690 |aViện Kỹ thuật Công nghệ cao NTT
773 |tTạp chí Khoa học và Công nghệ - Đại học Nguyễn Tất Thành |d2020|gtr. 06-11|x2615-9015|i12
890|a0|b0|c1|d31
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