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Khảo sát hàm lượng Flavonoid trong vỏ hạt một số giống đậu xanh bằng phương pháp HPLC / Nguyễn Đình Dũng, Nguyễn Thu Hằng, Nguyễn Duy Chi, Hứa Thùy Linh // Tạp chí Dược học . - 2018. - tr. 23-28. - ISSN:
Ký hiệu phân loại (DDC): 615
The flavonoid Isovitexin and Vitexin in Vigna radiata (L.) R.Wilczeck. were analysed by HPLC. The method was established as: Column – Phenomenex (C18, 150 x 4,6 mm; 5µm); Mobile phase - 0.1% orthophosphoric acid in water (w/v) : Methanol (65:35); Flow- rate - 1.0ml/min. The method was validated over the concentration range of 5µg/ml-150µg/ml of Isovitexin and Vitexin each for the linearity (quite satisfactory, r=0.9996 and 0.9999, respectively), limit of detection (LOD=0.024µg/ml and 0.021µg/ml) and limit of quantification (LOQ=0.072µg/ml and 0.063µg/ml), recoveries (desirable > 99%). The content of Isovitexin and Vitexin in most samples collected in the market largely varied from one another, ranging from 3.57- 5.8 mg/g for Isovitexin and from 4.8- 7.7mg/g for Vitexin.
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