DDC
| 571.6 |
Tác giả CN
| Nguyen, Thi Phuong |
Nhan đề
| An effective protocol for isolation and culture of mesenchymal stem cells from mouse bone marrow / Nguyen Thi Phuong, Nguyen Huu Hung |
Thông tin xuất bản
| Ho Chi Minh city : Nguyen Tat Thanh University, 2018 |
Mô tả vật lý
| 5 p. |
Tóm tắt
| Bone marrow is known as the main source of isolated mesenchymal stem cells. This study has demonstrated a protocol for isolation and culture of mesenchymal stem cells from mouse bone marrow (mBM) with minimal requirement. The cells were harvested from the tibias and femurs, and then cultured in Dulbecco’s modified Eagle’s medium supplemented with fetal bovine serum. The mBM-MSCs were isolated by media changing every 8 hours in 24 hours of culture and sub-culture. Target cells subsequently were regconized by adipogenic differentiaton posibility. The study also focused on descript a simple procedure to culture BM-MSCs and the results obtained following the procedure |
Từ khóa tự do
| Nuôi cấy tế bào |
Từ khóa tự do
| Isolation |
Từ khóa tự do
| Tế bào gốc trung mô |
Từ khóa tự do
| Bone marrow |
Từ khóa tự do
| Cell culture |
Từ khóa tự do
| Differentiation |
Từ khóa tự do
| Tủy xương |
Nguồn trích
| Tạp chí Khoa học và Công nghệ - Đại học Nguyễn Tất Thành 2018tr. 18-22
Số: 02 |
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001 | 29295 |
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002 | 9 |
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004 | C09D07D6-9724-46FF-AD61-50D331C5EECF |
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005 | 202309081050 |
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008 | 081223s2018 vm| vie |
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009 | 1 0 |
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039 | |a20230908105003|bbacntp|y20210624112139|zngantk |
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082 | |a571.6 |
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100 | |aNguyen, Thi Phuong |
---|
245 | |aAn effective protocol for isolation and culture of mesenchymal stem cells from mouse bone marrow / |cNguyen Thi Phuong, Nguyen Huu Hung |
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260 | |aHo Chi Minh city : |bNguyen Tat Thanh University, |c2018 |
---|
300 | |a5 p. |
---|
520 | |aBone marrow is known as the main source of isolated mesenchymal stem cells. This study has demonstrated a protocol for isolation and culture of mesenchymal stem cells from mouse bone marrow (mBM) with minimal requirement. The cells were harvested from the tibias and femurs, and then cultured in Dulbecco’s modified Eagle’s medium supplemented with fetal bovine serum. The mBM-MSCs were isolated by media changing every 8 hours in 24 hours of culture and sub-culture. Target cells subsequently were regconized by adipogenic differentiaton posibility. The study also focused on descript a simple procedure to culture BM-MSCs and the results obtained following the procedure |
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653 | |aNuôi cấy tế bào |
---|
653 | |aIsolation |
---|
653 | |aTế bào gốc trung mô |
---|
653 | |aBone marrow |
---|
653 | |aCell culture |
---|
653 | |aDifferentiation |
---|
653 | |aTủy xương |
---|
690 | |aKhoa Công nghệ sinh học |
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773 | |tTạp chí Khoa học và Công nghệ - Đại học Nguyễn Tất Thành |d2018|gtr. 18-22|x2615-9015|i02 |
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890 | |a0|b0|c1|d21 |
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