thông tin biểu ghi
  • Bài trích
  • Ký hiệu PL/XG: 571.6
    Nhan đề: An effective protocol for isolation and culture of mesenchymal stem cells from mouse bone marrow /

DDC 571.6
Tác giả CN Nguyen, Thi Phuong
Nhan đề An effective protocol for isolation and culture of mesenchymal stem cells from mouse bone marrow / Nguyen Thi Phuong, Nguyen Huu Hung
Thông tin xuất bản Ho Chi Minh city : Nguyen Tat Thanh University, 2018
Mô tả vật lý 5 p.
Tóm tắt Bone marrow is known as the main source of isolated mesenchymal stem cells. This study has demonstrated a protocol for isolation and culture of mesenchymal stem cells from mouse bone marrow (mBM) with minimal requirement. The cells were harvested from the tibias and femurs, and then cultured in Dulbecco’s modified Eagle’s medium supplemented with fetal bovine serum. The mBM-MSCs were isolated by media changing every 8 hours in 24 hours of culture and sub-culture. Target cells subsequently were regconized by adipogenic differentiaton posibility. The study also focused on descript a simple procedure to culture BM-MSCs and the results obtained following the procedure
Từ khóa tự do Nuôi cấy tế bào
Từ khóa tự do Isolation
Từ khóa tự do Tế bào gốc trung mô
Từ khóa tự do Bone marrow
Từ khóa tự do Cell culture
Từ khóa tự do Differentiation
Từ khóa tự do Tủy xương
Nguồn trích Tạp chí Khoa học và Công nghệ - Đại học Nguyễn Tất Thành 2018tr. 18-22 Số: 02
000 00000nab#a2200000ui#4500
00129295
0029
004C09D07D6-9724-46FF-AD61-50D331C5EECF
005202309081050
008081223s2018 vm| vie
0091 0
039|a20230908105003|bbacntp|y20210624112139|zngantk
082 |a571.6
100 |aNguyen, Thi Phuong
245 |aAn effective protocol for isolation and culture of mesenchymal stem cells from mouse bone marrow / |cNguyen Thi Phuong, Nguyen Huu Hung
260 |aHo Chi Minh city : |bNguyen Tat Thanh University, |c2018
300 |a5 p.
520 |aBone marrow is known as the main source of isolated mesenchymal stem cells. This study has demonstrated a protocol for isolation and culture of mesenchymal stem cells from mouse bone marrow (mBM) with minimal requirement. The cells were harvested from the tibias and femurs, and then cultured in Dulbecco’s modified Eagle’s medium supplemented with fetal bovine serum. The mBM-MSCs were isolated by media changing every 8 hours in 24 hours of culture and sub-culture. Target cells subsequently were regconized by adipogenic differentiaton posibility. The study also focused on descript a simple procedure to culture BM-MSCs and the results obtained following the procedure
653 |aNuôi cấy tế bào
653 |aIsolation
653 |aTế bào gốc trung mô
653 |aBone marrow
653 |aCell culture
653 |aDifferentiation
653 |aTủy xương
690 |aKhoa Công nghệ sinh học
773 |tTạp chí Khoa học và Công nghệ - Đại học Nguyễn Tất Thành |d2018|gtr. 18-22|x2615-9015|i02
890|a0|b0|c1|d21
Không tìm thấy biểu ghi nào