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DDC
| 664.022 | |
Tác giả CN
| Phung, Thi Thu Huong | |
Nhan đề
| Purification of Saccharomyces cerevisiae recombinant Crp1 / Phung Thi Thu Huong, Tran Hong Diem | |
Thông tin xuất bản
| Tp. Hồ Chí Minh : Đại học Nguyễn Tất Thành, 2020 | |
Mô tả vật lý
| 5 tr. | |
Tóm tắt
| A complex Mus81-Mm4 is a DNA structure–specific endonuclease in Saccharomyces cerevisiae. Mus81-Mms4 functions in processing of recombination intermediates that could arise during the repair of stalled and blocked replication forks and double stranded breaks. Mus81-Mms4 works with many proteins involved in DNA repair, replication fork stability, and joint molecule formation/resolution during homologous recombination repair. A biochemical screening of protein(s) that enhances the Mus81-Mms4 endonuclease activity on its preferable substrates in vitro revealed that Crp1, a cruciform DNA-recognizing protein, which can specifically bind to DNA four-way junction structures like Holliday junctions could be the potential factor. To further demonstrate that Crp1 interacts functionally with Mus81-Mms4 in vitro, we carried out the purification of recombinant Crp1 using Escherichia coli system. Our results showed that the purified Crp1 was highly homogenous and active that is ready for biochemical use. | |
Từ khóa tự do
| Purification | |
Từ khóa tự do
| Crpl | |
Từ khóa tự do
| DNA binding | |
Từ khóa tự do
| Mus81 | |
Nguồn trích
| Tạp chí Khoa học và Công nghệ - Đại học Nguyễn Tất Thành 2018tr. 22-26
Số: 04 |
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000
| 00000nab#a2200000ui#4500 |
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| 001 | 28816 |
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| 002 | 9 |
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| 004 | 978A461D-5F06-426D-840A-29B501601584 |
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| 005 | 202106090921 |
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| 008 | 081223s2020 vm| vie |
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| 009 | 1 0 |
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| 039 | |y20210609092123|zngantk |
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| 082 | |a664.022 |
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| 100 | |aPhung, Thi Thu Huong |
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| 245 | |aPurification of Saccharomyces cerevisiae recombinant Crp1 / |cPhung Thi Thu Huong, Tran Hong Diem |
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| 260 | |aTp. Hồ Chí Minh : |bĐại học Nguyễn Tất Thành, |c2020 |
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| 300 | |a5 tr. |
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| 520 | |aA complex Mus81-Mm4 is a DNA structure–specific endonuclease in Saccharomyces cerevisiae. Mus81-Mms4 functions in processing of recombination intermediates that could arise during the repair of stalled and blocked replication forks and double stranded breaks. Mus81-Mms4 works with many proteins involved in DNA repair, replication fork stability, and joint molecule formation/resolution during homologous recombination repair. A biochemical screening of protein(s) that enhances the Mus81-Mms4 endonuclease activity on its preferable substrates in vitro revealed that Crp1, a cruciform DNA-recognizing protein, which can specifically bind to DNA four-way junction structures like Holliday junctions could be the potential factor. To further demonstrate that Crp1 interacts functionally with Mus81-Mms4 in vitro, we carried out the purification of recombinant Crp1 using Escherichia coli system. Our results showed that the purified Crp1 was highly homogenous and active that is ready for biochemical use. |
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| 653 | |aPurification |
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| 653 | |aCrpl |
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| 653 | |aDNA binding |
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| 653 | |aMus81 |
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| 690 | |aViện Kỹ thuật Công nghệ cao NTT |
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| 773 | |tTạp chí Khoa học và Công nghệ - Đại học Nguyễn Tất Thành |d2018|gtr. 22-26|x2615-9015|i04 |
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| 890 | |a0|b0|c1|d38 |
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