|
DDC
| 660 | |
Tác giả CN
| Ho, Ta Giap | |
Nhan đề
| Cloning of an AA13 Polysaccharide Monooxygenase Gene from Neurospora crassa into the plasmid pEX2B / Ho Ta Giap, Ngo Nguyen Vu, Vu Van Van | |
Thông tin xuất bản
| Ho Chi Minh City : Nguyen Tat Thanh University, 2020 | |
Mô tả vật lý
| 6 p. | |
Tóm tắt
| Polysaccharide monooxygenases (PMOs) cleave glycoside linkages in polysaccharides, which has great potential in manufacturing biofuel from polysaccharide biomasses. Therefore, heterologous production of PMOs is required for the development of their application. We aim to clone PMO genes into the plasmid pEX2B to express PMOs in Aspergillus oryzae, a filamentous fungus widely used for industrial enzyme production. Here we cloned the NCU08746 gene, an
AA13 PMO from Neurospora crassa, into pEX2B. We successfully designed primers and amplified the NCU08746 gene from the N. crassa genome. NCU08746 was then cloned into the pEX2B vector. The recombinant plasmid pEX2B-NCU08746 was subsequently transferred into A. tumefaciens AGL1 prior to being transferred into A. oryzae. This work provides the foundation for further study on PMOs expression in A. oryzae. | |
Từ khóa tự do
| AA13 | |
Từ khóa tự do
| Agrobacterium tumefaciens | |
Từ khóa tự do
| monooxygenases | |
Từ khóa tự do
| NCU08746 | |
Từ khóa tự do
| Neurospora crassa | |
Từ khóa tự do
| Polysaccharide | |
Tác giả(bs) CN
| Ngo, Nguyen Vu | |
Tác giả(bs) CN
| Vu, Van Van | |
Nguồn trích
| Tạp chí Khoa học và Công nghệ - Đại học Nguyễn Tất Thành 2020p. 13-18
Số: 11 |
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| 00000nab#a2200000ui#4500 |
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| 001 | 27651 |
|---|
| 002 | 9 |
|---|
| 004 | F79F525E-41E0-49A9-80A6-FFE837751CDA |
|---|
| 005 | 202104021130 |
|---|
| 008 | 081223s2020 vm| vie |
|---|
| 009 | 1 0 |
|---|
| 039 | |a20210402113007|btainguyendientu|c20210402112929|dtainguyendientu|y20210324172201|ztainguyendientu |
|---|
| 082 | |a660 |
|---|
| 100 | |aHo, Ta Giap |
|---|
| 245 | |aCloning of an AA13 Polysaccharide Monooxygenase Gene from Neurospora crassa into the plasmid pEX2B / |cHo Ta Giap, Ngo Nguyen Vu, Vu Van Van |
|---|
| 260 | |aHo Chi Minh City : |bNguyen Tat Thanh University, |c2020 |
|---|
| 300 | |a6 p. |
|---|
| 520 | |aPolysaccharide monooxygenases (PMOs) cleave glycoside linkages in polysaccharides, which has great potential in manufacturing biofuel from polysaccharide biomasses. Therefore, heterologous production of PMOs is required for the development of their application. We aim to clone PMO genes into the plasmid pEX2B to express PMOs in Aspergillus oryzae, a filamentous fungus widely used for industrial enzyme production. Here we cloned the NCU08746 gene, an
AA13 PMO from Neurospora crassa, into pEX2B. We successfully designed primers and amplified the NCU08746 gene from the N. crassa genome. NCU08746 was then cloned into the pEX2B vector. The recombinant plasmid pEX2B-NCU08746 was subsequently transferred into A. tumefaciens AGL1 prior to being transferred into A. oryzae. This work provides the foundation for further study on PMOs expression in A. oryzae. |
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| 653 | |aAA13 |
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| 653 | |aAgrobacterium tumefaciens |
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| 653 | |amonooxygenases |
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| 653 | |aNCU08746 |
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| 653 | |aNeurospora crassa |
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| 653 | |aPolysaccharide |
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| 700 | |aNgo, Nguyen Vu |
|---|
| 700 | |aVu, Van Van |
|---|
| 773 | |tTạp chí Khoa học và Công nghệ - Đại học Nguyễn Tất Thành |d2020|gp. 13-18|x2615-9015|i11 |
|---|
| 890 | |c1|a0|b0|d29 |
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