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  • Bài trích
  • Ký hiệu PL/XG: 660
    Nhan đề: Cloning of an AA13 Polysaccharide Monooxygenase Gene from Neurospora crassa into the plasmid pEX2B /
DDC 660
Tác giả CN Ho, Ta Giap
Nhan đề Cloning of an AA13 Polysaccharide Monooxygenase Gene from Neurospora crassa into the plasmid pEX2B / Ho Ta Giap, Ngo Nguyen Vu, Vu Van Van
Thông tin xuất bản Ho Chi Minh City : Nguyen Tat Thanh University, 2020
Mô tả vật lý 6 p.
Tóm tắt Polysaccharide monooxygenases (PMOs) cleave glycoside linkages in polysaccharides, which has great potential in manufacturing biofuel from polysaccharide biomasses. Therefore, heterologous production of PMOs is required for the development of their application. We aim to clone PMO genes into the plasmid pEX2B to express PMOs in Aspergillus oryzae, a filamentous fungus widely used for industrial enzyme production. Here we cloned the NCU08746 gene, an AA13 PMO from Neurospora crassa, into pEX2B. We successfully designed primers and amplified the NCU08746 gene from the N. crassa genome. NCU08746 was then cloned into the pEX2B vector. The recombinant plasmid pEX2B-NCU08746 was subsequently transferred into A. tumefaciens AGL1 prior to being transferred into A. oryzae. This work provides the foundation for further study on PMOs expression in A. oryzae.
Từ khóa tự do AA13
Từ khóa tự do Agrobacterium tumefaciens
Từ khóa tự do monooxygenases
Từ khóa tự do NCU08746
Từ khóa tự do Neurospora crassa
Từ khóa tự do Polysaccharide
Tác giả(bs) CN Ngo, Nguyen Vu
Tác giả(bs) CN Vu, Van Van
Nguồn trích Tạp chí Khoa học và Công nghệ - Đại học Nguyễn Tất Thành 2020p. 13-18 Số: 11
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039|a20210402113007|btainguyendientu|c20210402112929|dtainguyendientu|y20210324172201|ztainguyendientu
082|a660
100 |aHo, Ta Giap
245 |aCloning of an AA13 Polysaccharide Monooxygenase Gene from Neurospora crassa into the plasmid pEX2B / |cHo Ta Giap, Ngo Nguyen Vu, Vu Van Van
260 |aHo Chi Minh City : |bNguyen Tat Thanh University, |c2020
300 |a6 p.
520 |aPolysaccharide monooxygenases (PMOs) cleave glycoside linkages in polysaccharides, which has great potential in manufacturing biofuel from polysaccharide biomasses. Therefore, heterologous production of PMOs is required for the development of their application. We aim to clone PMO genes into the plasmid pEX2B to express PMOs in Aspergillus oryzae, a filamentous fungus widely used for industrial enzyme production. Here we cloned the NCU08746 gene, an AA13 PMO from Neurospora crassa, into pEX2B. We successfully designed primers and amplified the NCU08746 gene from the N. crassa genome. NCU08746 was then cloned into the pEX2B vector. The recombinant plasmid pEX2B-NCU08746 was subsequently transferred into A. tumefaciens AGL1 prior to being transferred into A. oryzae. This work provides the foundation for further study on PMOs expression in A. oryzae.
653 |aAA13
653 |aAgrobacterium tumefaciens
653 |amonooxygenases
653 |aNCU08746
653 |aNeurospora crassa
653 |aPolysaccharide
700 |aNgo, Nguyen Vu
700 |aVu, Van Van
773 |tTạp chí Khoa học và Công nghệ - Đại học Nguyễn Tất Thành |d2020|gp. 13-18|x2615-9015|i11
890|c1|a0|b0|d29
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