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DDC
| 615.321 | |
Nhan đề
| Review Advances in-capillary electrophoretic enzyme assays / Yi Fan, Gerhard K.E. Scriba | |
Thông tin xuất bản
| Amsterdam ; Oxford : Elsevier JAI, 2010 | |
Mô tả vật lý
| 15 p. : illustrations | |
Tóm tắt
| In recent years, capillary electrophoresis (CE) has become a frequently used tool for enzyme assays due to its well-recognized advantages such as high separation efficiency, short analysis time, small sample and micals consumption. The published applications cover all pects of enzyme characterization and analysis including the determination of the enzyme activity, substrate and lator characterization and identification, as well as the igation of enzyme-mediated metabolic pathways of bioactive molecules. The CE assays may be classified into two general categories: (1) pre-capillary assays where the reactions are performed offline followed by CE analysis of the substrates and products and (2) online assays when the enzyme reaction and separation of the nalytes are performed in the same capillary. In online assays, the enzyme may be either immobilized or in solution. The latter is also referred to as electrophoretically mediated microanalysis (EMMA). The present review will highlight the literature of CE-based enzyme assays from 2006 to November 2009. One section will be devoted to applications of microfluidic devices. | |
Từ khóa tự do
| Enzyme assay | |
Từ khóa tự do
| Capillary electrophoresis | |
Từ khóa tự do
| Electrophoretically mediated microanalysis | |
Từ khóa tự do
| Microfluidic device | |
Tác giả(bs) CN
| Scriba, Gerhard K.E. | |
Nguồn trích
| Journal of Pharmaceutical and Biomedical AnalysisVolume 53, Issue 5, 15 December 2010, pp. 1076-1090 | |
Địa chỉ
| Thư Viện Đại học Nguyễn Tất Thành | |
Tệp tin điện tử
| https://doi.org/10.1016/j.jpba.2010.04.005 | |
Tệp tin điện tử
| http://elib.ntt.edu.vn/documentdata01/6 baibaokhcn/anhbia/27547_reviewadvancesinceenzymeassays_kthumbimage.jpg |
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| 082 | |a615.321|bF1991Y|223 |
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| 245 | |aReview Advances in-capillary electrophoretic enzyme assays / |cYi Fan, Gerhard K.E. Scriba |
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| 260 | |aAmsterdam ; Oxford : |bElsevier JAI, |c2010 |
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| 300 | |a15 p. : |billustrations |
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| 520 | |aIn recent years, capillary electrophoresis (CE) has become a frequently used tool for enzyme assays due to its well-recognized advantages such as high separation efficiency, short analysis time, small sample and micals consumption. The published applications cover all pects of enzyme characterization and analysis including the determination of the enzyme activity, substrate and lator characterization and identification, as well as the igation of enzyme-mediated metabolic pathways of bioactive molecules. The CE assays may be classified into two general categories: (1) pre-capillary assays where the reactions are performed offline followed by CE analysis of the substrates and products and (2) online assays when the enzyme reaction and separation of the nalytes are performed in the same capillary. In online assays, the enzyme may be either immobilized or in solution. The latter is also referred to as electrophoretically mediated microanalysis (EMMA). The present review will highlight the literature of CE-based enzyme assays from 2006 to November 2009. One section will be devoted to applications of microfluidic devices. |
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| 653 | |aEnzyme assay |
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| 653 | |aCapillary electrophoresis |
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| 653 | |aElectrophoretically mediated microanalysis |
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| 653 | |aMicrofluidic device |
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| 690 | |aKhoa Dược |
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| 700 | |aScriba, Gerhard K.E. |
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| 773 | |tJournal of Pharmaceutical and Biomedical Analysis|gVolume 53, Issue 5, 15 December 2010, pp. 1076-1090 |
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| 852 | |aThư Viện Đại học Nguyễn Tất Thành |
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| 856 | |uhttps://doi.org/10.1016/j.jpba.2010.04.005 |
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