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  • Ký hiệu PL/XG: 615
    Nhan đề: Định lượng Domperidon trong huyết tương người bằng phương pháp sắc ký lỏng siêu hiệu năng ghép nối với Detector khối phổ (UPLC-MS/MS) /
DDC 615
Tác giả CN Tạ, Mạnh Hùng
Nhan đề Định lượng Domperidon trong huyết tương người bằng phương pháp sắc ký lỏng siêu hiệu năng ghép nối với Detector khối phổ (UPLC-MS/MS) / Tạ Mạnh Hùng, Phạm Thanh Huyền, Phan Thị Nghĩa
Tóm tắt An ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was developed for determination of domperidone in human plasma with diphenhydramine as internal standard: Column - C18 (150 x 4.6mm, i.d., 5 µm); mobile phase - 0.05% formic acid - methanol - acetonitrile - isopropanol (suitable ratio); flow rate - 0.5 ml/min (isocratic elution). The UPLC-MS/MS was operated in the multi reaction-monitoring mode using the electrospray ionization technique. For injection samples, domperidone and the internal standard (diphenhydramine) were extracted from human plasma by liquid-liquid extraction with a mixture of diethylether and chloroform. Good linearity for standard curves was found at the range of 0.5 to 50 ng/ml for domperidone (mean correlation coefficient R2 ≥ 0.99). The intra-day and inter-day precision and accuracy were within 88.8% - 106.8%. The method was excellently time-saving - the total run time was 4 min only. This method showed applicable to BA-BE studies of domperidone preparations.
Thuật ngữ chủ đề Nghiên cứu kỹ thuật --Việt Nam
Từ khóa tự do Huyết tương
Từ khóa tự do Detector
Từ khóa tự do Domperidon
Tác giả(bs) CN Phạm, Thanh Huyền
Nguồn trích Tạp chí Dược học 2018tr. 29-34 Số: 07
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005201912061508
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039|y20191206150828|zthienvan
082 |a615
100 |aTạ, Mạnh Hùng
245 |aĐịnh lượng Domperidon trong huyết tương người bằng phương pháp sắc ký lỏng siêu hiệu năng ghép nối với Detector khối phổ (UPLC-MS/MS) / |cTạ Mạnh Hùng, Phạm Thanh Huyền, Phan Thị Nghĩa
520 |aAn ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was developed for determination of domperidone in human plasma with diphenhydramine as internal standard: Column - C18 (150 x 4.6mm, i.d., 5 µm); mobile phase - 0.05% formic acid - methanol - acetonitrile - isopropanol (suitable ratio); flow rate - 0.5 ml/min (isocratic elution). The UPLC-MS/MS was operated in the multi reaction-monitoring mode using the electrospray ionization technique. For injection samples, domperidone and the internal standard (diphenhydramine) were extracted from human plasma by liquid-liquid extraction with a mixture of diethylether and chloroform. Good linearity for standard curves was found at the range of 0.5 to 50 ng/ml for domperidone (mean correlation coefficient R2 ≥ 0.99). The intra-day and inter-day precision and accuracy were within 88.8% - 106.8%. The method was excellently time-saving - the total run time was 4 min only. This method showed applicable to BA-BE studies of domperidone preparations.
650 |aNghiên cứu kỹ thuật |zViệt Nam
653 |aHuyết tương
653 |aDetector
653 |aDomperidon
700 |aPhạm, Thanh Huyền
773 |tTạp chí Dược học |d2018|gtr. 29-34|i07
890|c1|a0|b0|d5
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