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  • Bài trích
  • Ký hiệu PL/XG: 615
    Nhan đề: Phân tích định tính và định lượng Flavonoid trong thân rễ Ngải đen (Kaempferia Parviflora Wall. ex. Baker ) bằng sắc ký lớp mỏng và phương pháp quang phổ /
DDC 615
Tác giả CN Đỗ, Thị Hà
Nhan đề Phân tích định tính và định lượng Flavonoid trong thân rễ Ngải đen (Kaempferia Parviflora Wall. ex. Baker ) bằng sắc ký lớp mỏng và phương pháp quang phổ / Đỗ Thị Hà, Vũ Thị Diệp, Nguyễn Thị Thu, Nguyễn Tiễn Dũng, Nguyễn Thị Thu, Trần Ngọc Lân
Tóm tắt Flavonoids in the rhizomes of Kaempferia parviflora (Wall. ex. Baker) cultivated in Vietnam were determined: 5,7-dimethoxyflavone by thin-layer chromatography (TLC) and the total flavonoids by spectrophotometry, both with 5,7-dimethoxyflavone as reference. For qualitative TLC, the samples were prepared as follows: to 1.0 g of the ground material, 10 ml methanol was added, kept ultrasonicated for 1 h, filtered to get the filtrate for test solution. TLC was performed as: TLC plate - Silica gel 60 GF254; Developing solvent - a mixture of toluen – chloroform – aceton – methanol – acid formic (5:4:0,1:0.2:0.2, v/v/v/v/v). The total flavonoids were extracted with 96 % ethanol and Quantitatively determined by UV-spectrophotometry (265 nm) with 5,7-dimethoxyflavone as reference. The method was validated in terms of linearity (r2 = 0.9965), repeatability (RSD = 1.73 %) and accuracy (98.60 % - 100.55 %). The total flavonoids content of the samples collected from three provinces of Vietnam (Nghe An, Thanh Hoa and Son La) were found to be 4.10 – 5.91 % calculated on the dried materials.
Thuật ngữ chủ đề Nghiên cứu kỹ thuật-- Việt Nam
Từ khóa tự do Flavonoid
Từ khóa tự do Ngải đen
Từ khóa tự do Sắc ký lớp mỏng
Tác giả(bs) CN Vũ, Thị Diệp
Nguồn trích Tạp chí Dược học 2018tr. 25-29 Số: 12
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039|y20191204154556|zthienvan
082 |a615
100 |aĐỗ, Thị Hà
245 |aPhân tích định tính và định lượng Flavonoid trong thân rễ Ngải đen (Kaempferia Parviflora Wall. ex. Baker ) bằng sắc ký lớp mỏng và phương pháp quang phổ / |cĐỗ Thị Hà, Vũ Thị Diệp, Nguyễn Thị Thu, Nguyễn Tiễn Dũng, Nguyễn Thị Thu, Trần Ngọc Lân
520 |aFlavonoids in the rhizomes of Kaempferia parviflora (Wall. ex. Baker) cultivated in Vietnam were determined: 5,7-dimethoxyflavone by thin-layer chromatography (TLC) and the total flavonoids by spectrophotometry, both with 5,7-dimethoxyflavone as reference. For qualitative TLC, the samples were prepared as follows: to 1.0 g of the ground material, 10 ml methanol was added, kept ultrasonicated for 1 h, filtered to get the filtrate for test solution. TLC was performed as: TLC plate - Silica gel 60 GF254; Developing solvent - a mixture of toluen – chloroform – aceton – methanol – acid formic (5:4:0,1:0.2:0.2, v/v/v/v/v). The total flavonoids were extracted with 96 % ethanol and Quantitatively determined by UV-spectrophotometry (265 nm) with 5,7-dimethoxyflavone as reference. The method was validated in terms of linearity (r2 = 0.9965), repeatability (RSD = 1.73 %) and accuracy (98.60 % - 100.55 %). The total flavonoids content of the samples collected from three provinces of Vietnam (Nghe An, Thanh Hoa and Son La) were found to be 4.10 – 5.91 % calculated on the dried materials.
650 |aNghiên cứu kỹ thuật|z Việt Nam
653 |aFlavonoid
653 |aNgải đen
653 |aSắc ký lớp mỏng
700 |aVũ, Thị Diệp
773 |tTạp chí Dược học |d2018|gtr. 25-29|i12
890|c1|a0|b0|d10
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