|
DDC
| 615 | |
Tác giả CN
| Nguyễn, Thị Dung | |
Nhan đề
| Phân tích atorvastatin và chất chuyển hóa trong huyết tương người bằng phương pháp sắc ký lỏng siêu hiệu năng ghép nối với Detector khối phổ / Nguyễn Thị Dung, Phạm Thanh Huyền, Phan Thị Nghĩa, Tạ Mạnh Hùng | |
Tóm tắt
| An ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was developed for determination of atorvastatin and its two active metabolites (ortho and para-hydroxy atorvastatin) in human plasma. The Thermo Vantage UPLC-MS/MS was operated in the multi reaction-monitoring mode using the electrospray ionization. Atorvastatin, o- and p-hydroxy atorvastatin and the internal standard (glibenclamide) were extracted from human plasma by liquid - liquid extraction. The reconstituted samples were chromatographed as follows: Column - C18 column (100 x 2.1 mm, i.d., 1.7 µm); mobile phase - 0.001% formic acid - methanol; isocratic elution, the flow rate of 0.2 ml/min. The standard curves showed good linearity (correlation coefficient R2 ≥ 0.99) at the concentration range of 0.1 - 20 ng/ml for atorvastatin, 0.05 - 10 ng/ml for o-hydroxy atorvastatin and 0.01 – 2 ng/ml for p-hydroxy atorvastatin. The intra-day, inter-day precision and accuracy ranged at 90% - 110.0%. As thus, the proposed method proved applicable to BA-BE studies of atorvastatin preparations. | |
Thuật ngữ chủ đề
| Nghiên cứu kỹ thuật--Việt Nam | |
Từ khóa tự do
| Huyết tương người | |
Từ khóa tự do
| Detector khối phổ | |
Tác giả(bs) CN
| Phạm, Thanh Huyền | |
Nguồn trích
| Tạp chí Dược học 2019tr. 22-27
Số: 01 |
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000
| 00000nab#a2200000ui#4500 |
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| 001 | 18583 |
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| 002 | 9 |
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| 004 | 470E3B0F-8184-4A6E-8390-AB98B8B6E7FB |
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| 005 | 201912041017 |
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| 008 | 081223s vm| vie |
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| 009 | 1 0 |
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| 039 | |y20191204101716|zthienvan |
|---|
| 082 | |a615 |
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| 100 | |aNguyễn, Thị Dung |
|---|
| 245 | |aPhân tích atorvastatin và chất chuyển hóa trong huyết tương người bằng phương pháp sắc ký lỏng siêu hiệu năng ghép nối với Detector khối phổ / |cNguyễn Thị Dung, Phạm Thanh Huyền, Phan Thị Nghĩa, Tạ Mạnh Hùng |
|---|
| 520 | |aAn ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was developed for determination of atorvastatin and its two active metabolites (ortho and para-hydroxy atorvastatin) in human plasma. The Thermo Vantage UPLC-MS/MS was operated in the multi reaction-monitoring mode using the electrospray ionization. Atorvastatin, o- and p-hydroxy atorvastatin and the internal standard (glibenclamide) were extracted from human plasma by liquid - liquid extraction. The reconstituted samples were chromatographed as follows: Column - C18 column (100 x 2.1 mm, i.d., 1.7 µm); mobile phase - 0.001% formic acid - methanol; isocratic elution, the flow rate of 0.2 ml/min. The standard curves showed good linearity (correlation coefficient R2 ≥ 0.99) at the concentration range of 0.1 - 20 ng/ml for atorvastatin, 0.05 - 10 ng/ml for o-hydroxy atorvastatin and 0.01 – 2 ng/ml for p-hydroxy atorvastatin. The intra-day, inter-day precision and accuracy ranged at 90% - 110.0%. As thus, the proposed method proved applicable to BA-BE studies of atorvastatin preparations. |
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| 650 | |aNghiên cứu kỹ thuật|zViệt Nam |
|---|
| 653 | |aHuyết tương người |
|---|
| 653 | |aDetector khối phổ |
|---|
| 700 | |aPhạm, Thanh Huyền |
|---|
| 773 | |tTạp chí Dược học |d2019|gtr. 22-27|i01 |
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| 890 | |c1|a0|b0|d12 |
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