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  • Ký hiệu PL/XG: 615
    Nhan đề: Định lượng đồng thời Metronidazol và Spiramycin I trong huyết tương người bằng LC-MS/MS /
DDC 615
Tác giả CN Trần, Thị Mỹ Dung
Nhan đề Định lượng đồng thời Metronidazol và Spiramycin I trong huyết tương người bằng LC-MS/MS / Trần Thị Mỹ Dung, Bùi Mỹ Hạnh, Chương Ngọc Nãi, Nguyễn Đức Tuấn
Tóm tắt An isocratic liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) was developed for simultaneous assay of metronidazole (MET) and spiramycin I (SPIY I) in human plasma. The blank human plasma with MET, SPY I, and roxithromycin (ROX) added as was internal standards was treated by protein precipitation with acetonitrile. The analytes were chromatographed as follows: Column - Phenomenex NX C18 column (100 mm x 3.0 mm; 5.0 µm); Mobile phase - acetonitrile and ammonium formate 5 mM pH 7.4 (80:20, v/v); Flow rate - 0.4 mL/min.; Injection volume - 3 µL; Column temperature - 40oC; Detector – MS in positive ion electrospray ionization (ESI+) with multi-reaction monitoring (MRM) mode; m/z 171.75 → 128.10 for MET, 422.25 → 174.20 for SPY I, and 837.50 → 679.45 for ROX. Linearity covered the concentration range of 0.050-25.235 and 0.025-12.725 µg/ml for MET and SPY I, respectively. The intra- and inter-day accuracy and precisions (CV) were from 95.40 % to 106.39 % with CV value below 15.0 % for both MET and SPY I. The method showed appliable to determination of pharmacokinetic parameters of MET and SPY I in human plasma.
Thuật ngữ chủ đề Nghiên cứu kỹ thuật--Việt Nam
Từ khóa tự do Huyết tương người
Từ khóa tự do Metronidazol
Từ khóa tự do Spiramycin
Tác giả(bs) CN Chương, Ngọc Nãi
Tác giả(bs) CN Bùi, Mỹ Hạnh
Nguồn trích Tạp chí Dược học 2019tr. 17-22 Số: 01
000 00000nab#a2200000ui#4500
00118567
0029
004F24FC9FA-06D7-4795-952C-DFDED80E2F6E
005201912040900
008081223s vm| vie
0091 0
039|y20191204090043|zthienvan
082 |a615
100 |aTrần, Thị Mỹ Dung
245 |aĐịnh lượng đồng thời Metronidazol và Spiramycin I trong huyết tương người bằng LC-MS/MS / |cTrần Thị Mỹ Dung, Bùi Mỹ Hạnh, Chương Ngọc Nãi, Nguyễn Đức Tuấn
520 |aAn isocratic liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) was developed for simultaneous assay of metronidazole (MET) and spiramycin I (SPIY I) in human plasma. The blank human plasma with MET, SPY I, and roxithromycin (ROX) added as was internal standards was treated by protein precipitation with acetonitrile. The analytes were chromatographed as follows: Column - Phenomenex NX C18 column (100 mm x 3.0 mm; 5.0 µm); Mobile phase - acetonitrile and ammonium formate 5 mM pH 7.4 (80:20, v/v); Flow rate - 0.4 mL/min.; Injection volume - 3 µL; Column temperature - 40oC; Detector – MS in positive ion electrospray ionization (ESI+) with multi-reaction monitoring (MRM) mode; m/z 171.75 → 128.10 for MET, 422.25 → 174.20 for SPY I, and 837.50 → 679.45 for ROX. Linearity covered the concentration range of 0.050-25.235 and 0.025-12.725 µg/ml for MET and SPY I, respectively. The intra- and inter-day accuracy and precisions (CV) were from 95.40 % to 106.39 % with CV value below 15.0 % for both MET and SPY I. The method showed appliable to determination of pharmacokinetic parameters of MET and SPY I in human plasma.
650 |aNghiên cứu kỹ thuật|zViệt Nam
653 |aHuyết tương người
653 |aMetronidazol
653 |aSpiramycin
700 |aChương, Ngọc Nãi
700 |aBùi, Mỹ Hạnh
773 |tTạp chí Dược học |d2019|gtr. 17-22|i01
890|c1|a0|b0|d12
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