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Nghiên cứu phân lập Andrographolid làm chất đối chiếu từ cây xuyên tâm liên (Andrographis paniculata (Burm.f.) Nees) / Lữ Thị Kim Chi, Huỳnh Tự Thuật, Nguyễn Ngọc Vinh // Tạp chí Dược học . - 2018. - tr. 49-52. - ISSN:
Ký hiệu phân loại (DDC): 615
Andrographolid, as a natural biological active, was isolated from Andrographis paniculata (Burm.f.) Nees (Acanthaceae) and purified to the purtity of 98.03%. Its structure was elucidated by UV-Vis, IR, MS, 1HNMR, 13CNMR. In addition, an HPLC method was validated for analysis of andrographolid. The calibration curves were established by six replicate experiments (with purified andrographolid) demontrated a good linear regression over a range of 120,06 – 283.13mg/ml (R2 = 0.9996); the precision RSD was less than 2%; the accuracy ranged from 99.01 – 101.24%. This proved the obtained andrographolid was fairly corresponding to the certified reference material of USP in terms of content, and therefore, may be established as reference standard for quality control and research of the plant.
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