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Định lượng Domperidon trong huyết tương người bằng phương pháp sắc ký lỏng siêu hiệu năng ghép nối với Detector khối phổ (UPLC-MS/MS) / Tạ Mạnh Hùng, Phạm Thanh Huyền, Phan Thị Nghĩa // Tạp chí Dược học . - 2018. - tr. 29-34. - ISSN:
Ký hiệu phân loại (DDC): 615
An ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was developed for determination of domperidone in human plasma with diphenhydramine as internal standard: Column - C18 (150 x 4.6mm, i.d., 5 µm); mobile phase - 0.05% formic acid - methanol - acetonitrile - isopropanol (suitable ratio); flow rate - 0.5 ml/min (isocratic elution). The UPLC-MS/MS was operated in the multi reaction-monitoring mode using the electrospray ionization technique. For injection samples, domperidone and the internal standard (diphenhydramine) were extracted from human plasma by liquid-liquid extraction with a mixture of diethylether and chloroform. Good linearity for standard curves was found at the range of 0.5 to 50 ng/ml for domperidone (mean correlation coefficient R2 ≥ 0.99). The intra-day and inter-day precision and accuracy were within 88.8% - 106.8%. The method was excellently time-saving - the total run time was 4 min only. This method showed applicable to BA-BE studies of domperidone preparations.
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Xây dựng phương pháp HPLC đơn giản để định lượng Cefaclor trong huyết tương hướng tới phục vụ theo dõi điều trị / Lê Đình Chi, Đào Danh Sơn // Tạp chí Dược học . - 2019. - tr. 28-31. - ISSN:
Ký hiệu phân loại (DDC): 615
An HPLC procedure was developed for quantitative determination of cefaclor in human plasma. For HPLC injection the analyzed plasma samples were treated by protein precipitation with trichloroacetic acid. The chromatography was performed with: Column - Zorbax Eclipse C18 (250 x 4.6 mm, 5 µm); Mobile phase - mixture of methanol-acetonitrile, aqueous solution of sodium dihydrogen phosphate 50 mM and tetrabutylammonium hydrogen sulfate 10 mM (pH 3.1) (16 : 9: 75, v/v/v); Flow rate (isocratic mode) - 1.0 ml/min; Detector – UV (263 nm.); injection volume - 100 ml. The method was fully validated observing the current conventional requirements and proving applicable to determinination of plasma cefaclor for therapeutic drug monitoring.
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