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Effects of Sodium Fluoride – Potassium Oxalate on Blood Lipid Profile Results and Lipid Stability when using this Anticoagulant : Xinh Thi Le and Ngoc Thi My Le // MedPharmRes . - 2023. - tr. 59-63. - ISSN: 2615-9139



Ký hiệu phân loại (DDC): 615.954
Introduction: Some previous studies have shown the effect of K2EDTA, heparin, citrate, kalioxalate on lipid test results [1,2,3], but no studies on sodium fluoride - potassium oxalate. Furthermore, it is also important to ensure sample stability during testing. We performed this study to evaluate the effect of NaF-KOx on lipid results and lipid stability when storing. Methods: A cross-sectional study was conducted on the residual of 150 pairs of serum and NaF-KOx plasma samples from the patients at the University Medical Center 2. These patients participated voluntarily after signing the consent form. We divided the samples into groups: serum (group 1), unpreserved plasma (group 2); stored plasma for 24 hours at 2-8°C (group 3), and stored plasma for 48 hours at 2-8°C (group 4). All samples were analyzed on an AU480 system – Beckman Coulter. The data would be processed using Stata 10.0. Results: The results difference between these groups: 2,3,4 compared to group 1 was statistically significant (p < 0.001). Total cholesterol, triglycerides, HDL-C, and LDL-C concentrations in group 2 decreased, respectively: 5.83%; 6.77%; 5.12%; 5.96%. We found the lipid test results of group 3 and group 4 also reduced compared to group 1: cholesterol decreased by 5.25%, 5.77%; triglycerides by 6.7%, 6.49%; HDL- C by 5.8, 7.22%; and LDL-C by 4.79%; 5.05%. After 48 hours, cholesterol, HDL-C, and LDL-C concentration continued to reduce, while the difference in triglycerides concentration was not statistically significant. Conclusions: NaF-KOx anticoagulant reduces lipid test results. We should not use NaF-KOx plasma to measure lipid tests.
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Evaluation of triglycerides, total cholesterol and high density lipoprotein-cholesterol stability in human whole blood and plasma samples / Thuan Thi Minh Nguyen, Dung Thi Van Nguyen // MedPharmRes . - 2024. - tr. 274-282. - ISSN: 2615-9139



Ký hiệu phân loại (DDC): 612.39
Introduction: Lipid tests are routinely performed in the laboratory for early diagnosis of lipid disorders and prevention of cardiovascular diseases. The aim of this study was to evaluate the stability of triglycerides (TGs), total cholesterol (TC), and high-density lipoprotein-cholesterol (HDL-C) in human whole blood (WB) and plasma samples stored at different temperature conditions in order to improve the quality of lipid testing. Methods: This cross-sectional study was conducted at Buon Ma Thuot Medical Testing Center. Ten mL of WB collected from 10 healthy volunteers from 18 to 60 years old were anticoagulated with dipotassium ethylenediaminetetraacetic acid. Five mL of WB were centrifuged to separate plasma. WB and plasma samples were stored at 20 ℃–25℃ and 2℃– 8 ℃. The concentrations of TGs, TC, and HDL-C in WB (WB-TGs, WB-TC, WB-HDL-C) and in plasma samples (P-TGs, P-TC, P-HDL-C) at each time point were determined simultaneously on Erba XL670 system. TGs, TC, and HDL-C were considered stable when the difference between concentrations at the later time point and baseline was not statistically significant. Results: At 20 ℃–25℃, the concentrations of WB-TGs, WB-TC, P-TGs, and P-TC were stable for less than 4 hours, while the concentrations of WB-HDL-C and P-HDL-C were stable for less than 12 hours. At 2 ℃–8℃, the concentrations of WB-TGs, WB-TC, WB-HDL-C, and P-HDL-C were stable for up to 48 hours, while the concentrations of P-TGs and P-TC were stable for up to 24 hours. Conclusions: The stability of TGs, TC, and HDL-C in WB and plasma samples was not the same at different tempera- tures.
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