Dòng Nội dung
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Assessment of stability of HbA1c levels in human whole blood samples using immunoassays / Thuan Thi Minh Nguyen, Dung Thi Van Nguyen // MedPharmRes . - 2023. - tr. 83-89. - ISSN: 2615-9139



Ký hiệu phân loại (DDC): 616.0756
Hemoglobin A1c (HbA1c) levels in whole blood samples are commonly used to diagnose diabetes and monitor the effectiveness of glycemic control. However, there have not been many studies evaluating changes in HbA1c concentration under different storage conditions and analytical methods. The purpose of this study was to evaluate the stability of %HbA1c stored at different temperature conditions using immunoassays in order to improve the quality of HbA1c test. Whole blood samples collected from 10 healthy volunteers were anticoagulated with K2 EDTA and stored at the following temperatures: 20-25 oC, 2-8 oC, and -20 oC. %HbA1c in human whole blood samples at each time point was determined simultaneously on Standard F Analyzer (%HbA1c-S) with reagent kit based on a reflectometry and immunoassay technology, and Erba XL640 system (%HbA1c-E) used immunoturbidimetric method, respectively. %HbA1c was assessed as stable when the difference in HbA1c level at the later time point was not statistically significant (p >0.05) compared with baseline (T0). Results showed that a positive correlation between %HbA1c-S and %HbA1c-E at T0 (r=0.9996) was observed at room temperature. %HbA1c-S was stabilized for 24 hours at 20-25 °C, for 2 days at 2-8°C and for more than 1 month at -20 °C. %HbA1c-E was stable for 12 hours at 20-25°C; less than 1 day at 2-8°C, and less than 1 month at -20°C. In conclusion, human whole blood samples for HbA1c determination can be stored for up to 1 month at -20°C.
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Handbook of immunoassay technologies : approaches, performances, and applications / Sandeep K. Vashist, John Luong (editor)
London : Academic Press, 2018
xvii, 478 pages. : illustrations (some color) ; 24 cm.
Ký hiệu phân loại (DDC): 616.4028
Handbook of Immunoassay Technologies: Approaches, Performances, and Applications unravels the role of immunoassays in the biochemical sciences. During the last four decades, a wide range of immunoassays has been developed, ranging from the conventional enzyme-linked immunosorbent assays, to the smartphone-based point-of-care formats. The advances in rapid biochemical procedures, novel biosensing schemes, fully integrated lab-on-a-chip platforms, prolonged biomolecular storage strategies, device miniaturization and interfacing, and emerging smart system technologies equipped with personalized mobile healthcare tools are paving the way to next-generation immunoassays, and are all discussed in this comprehensive text.
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The immunoassay handbook : theory and applications of ligand binding, ELISA and related techniques / David Wild (editor)
Oxford : Elsevier, 2013
xxi, 1013 pages. : illustrations ; 29 cm.
Ký hiệu phân loại (DDC): 616.0756
The Immunoassay Handbook provides an excellent, thoroughly updated guide to the science, technology and applications of ELISA and other immunoassays, including a wealth of practical advice. It encompasses a wide range of methods and gives an insight into the latest developments and applications in clinical and veterinary practice and in pharmaceutical and life science research.
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