Dòng Nội dung
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Assessment of stability of HbA1c levels in human whole blood samples using immunoassays / Thuan Thi Minh Nguyen, Dung Thi Van Nguyen // MedPharmRes . - 2023. - tr. 83-89. - ISSN: 2615-9139



Ký hiệu phân loại (DDC): 616.0756
Hemoglobin A1c (HbA1c) levels in whole blood samples are commonly used to diagnose diabetes and monitor the effectiveness of glycemic control. However, there have not been many studies evaluating changes in HbA1c concentration under different storage conditions and analytical methods. The purpose of this study was to evaluate the stability of %HbA1c stored at different temperature conditions using immunoassays in order to improve the quality of HbA1c test. Whole blood samples collected from 10 healthy volunteers were anticoagulated with K2 EDTA and stored at the following temperatures: 20-25 oC, 2-8 oC, and -20 oC. %HbA1c in human whole blood samples at each time point was determined simultaneously on Standard F Analyzer (%HbA1c-S) with reagent kit based on a reflectometry and immunoassay technology, and Erba XL640 system (%HbA1c-E) used immunoturbidimetric method, respectively. %HbA1c was assessed as stable when the difference in HbA1c level at the later time point was not statistically significant (p >0.05) compared with baseline (T0). Results showed that a positive correlation between %HbA1c-S and %HbA1c-E at T0 (r=0.9996) was observed at room temperature. %HbA1c-S was stabilized for 24 hours at 20-25 °C, for 2 days at 2-8°C and for more than 1 month at -20 °C. %HbA1c-E was stable for 12 hours at 20-25°C; less than 1 day at 2-8°C, and less than 1 month at -20°C. In conclusion, human whole blood samples for HbA1c determination can be stored for up to 1 month at -20°C.
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Evaluation of triglycerides, total cholesterol and high density lipoprotein-cholesterol stability in human whole blood and plasma samples / Thuan Thi Minh Nguyen, Dung Thi Van Nguyen // MedPharmRes . - 2024. - tr. 274-282. - ISSN: 2615-9139



Ký hiệu phân loại (DDC): 612.39
Introduction: Lipid tests are routinely performed in the laboratory for early diagnosis of lipid disorders and prevention of cardiovascular diseases. The aim of this study was to evaluate the stability of triglycerides (TGs), total cholesterol (TC), and high-density lipoprotein-cholesterol (HDL-C) in human whole blood (WB) and plasma samples stored at different temperature conditions in order to improve the quality of lipid testing. Methods: This cross-sectional study was conducted at Buon Ma Thuot Medical Testing Center. Ten mL of WB collected from 10 healthy volunteers from 18 to 60 years old were anticoagulated with dipotassium ethylenediaminetetraacetic acid. Five mL of WB were centrifuged to separate plasma. WB and plasma samples were stored at 20 ℃–25℃ and 2℃– 8 ℃. The concentrations of TGs, TC, and HDL-C in WB (WB-TGs, WB-TC, WB-HDL-C) and in plasma samples (P-TGs, P-TC, P-HDL-C) at each time point were determined simultaneously on Erba XL670 system. TGs, TC, and HDL-C were considered stable when the difference between concentrations at the later time point and baseline was not statistically significant. Results: At 20 ℃–25℃, the concentrations of WB-TGs, WB-TC, P-TGs, and P-TC were stable for less than 4 hours, while the concentrations of WB-HDL-C and P-HDL-C were stable for less than 12 hours. At 2 ℃–8℃, the concentrations of WB-TGs, WB-TC, WB-HDL-C, and P-HDL-C were stable for up to 48 hours, while the concentrations of P-TGs and P-TC were stable for up to 24 hours. Conclusions: The stability of TGs, TC, and HDL-C in WB and plasma samples was not the same at different tempera- tures.
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