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Định lượng Domperidon trong huyết tương người bằng phương pháp sắc ký lỏng siêu hiệu năng ghép nối với Detector khối phổ (UPLC-MS/MS) / Tạ Mạnh Hùng, Phạm Thanh Huyền, Phan Thị Nghĩa // Tạp chí Dược học . - 2018. - tr. 29-34. - ISSN:
Ký hiệu phân loại (DDC): 615
An ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was developed for determination of domperidone in human plasma with diphenhydramine as internal standard: Column - C18 (150 x 4.6mm, i.d., 5 µm); mobile phase - 0.05% formic acid - methanol - acetonitrile - isopropanol (suitable ratio); flow rate - 0.5 ml/min (isocratic elution). The UPLC-MS/MS was operated in the multi reaction-monitoring mode using the electrospray ionization technique. For injection samples, domperidone and the internal standard (diphenhydramine) were extracted from human plasma by liquid-liquid extraction with a mixture of diethylether and chloroform. Good linearity for standard curves was found at the range of 0.5 to 50 ng/ml for domperidone (mean correlation coefficient R2 ≥ 0.99). The intra-day and inter-day precision and accuracy were within 88.8% - 106.8%. The method was excellently time-saving - the total run time was 4 min only. This method showed applicable to BA-BE studies of domperidone preparations.
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Phân tích atorvastatin và chất chuyển hóa trong huyết tương người bằng phương pháp sắc ký lỏng siêu hiệu năng ghép nối với Detector khối phổ / Nguyễn Thị Dung, Phạm Thanh Huyền, Phan Thị Nghĩa, Tạ Mạnh Hùng // Tạp chí Dược học . - 2019. - tr. 22-27. - ISSN:
Ký hiệu phân loại (DDC): 615
An ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was developed for determination of atorvastatin and its two active metabolites (ortho and para-hydroxy atorvastatin) in human plasma. The Thermo Vantage UPLC-MS/MS was operated in the multi reaction-monitoring mode using the electrospray ionization. Atorvastatin, o- and p-hydroxy atorvastatin and the internal standard (glibenclamide) were extracted from human plasma by liquid - liquid extraction. The reconstituted samples were chromatographed as follows: Column - C18 column (100 x 2.1 mm, i.d., 1.7 µm); mobile phase - 0.001% formic acid - methanol; isocratic elution, the flow rate of 0.2 ml/min. The standard curves showed good linearity (correlation coefficient R2 ≥ 0.99) at the concentration range of 0.1 - 20 ng/ml for atorvastatin, 0.05 - 10 ng/ml for o-hydroxy atorvastatin and 0.01 – 2 ng/ml for p-hydroxy atorvastatin. The intra-day, inter-day precision and accuracy ranged at 90% - 110.0%. As thus, the proposed method proved applicable to BA-BE studies of atorvastatin preparations.
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