Dòng Nội dung
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Cloning human Benchwarmer gene (BNCH) harboring E164K in vector pcDNA3.1 by site-directed mutagenesis method / Nguyen Hoang Danh, Vu Minh Thiet // Tạp chí Khoa học và Công nghệ - Đại học Nguyễn Tất Thành . - 2020. - tr. 06-11. - ISSN: 2615-9015

Ho Chi Minh city : Nguyen Tat Thanh University, 2020
6 p.
Ký hiệu phân loại (DDC): 660.6
Benchwarmer (BNCH) gene encodes an orphan transmembrane transporter belonging to the Major Facilitator Superfamily (MFS), facilitating the transport of ions, amino acids, simple sugars and recently lysolipids. The loss of BNCH function caused lethality in several animal models with neurodegeneration and senescence. At the cellular level, dysregulation of BNCH leads to adverse phenotypes of lysosome and also autophagy (i.e. dyshomeostasis, accumulation of carbohydrates and sphingolipids, and enlarged lysosome). However, the molecular function and ligand of BNCH protein remain to be unrevealed. This study aims to create a radical substitution change in human BNCH coding gene to knock out the protein functions. More specifically, lysine (K) was used to replace the glutamic acid residue 164 (E164K) which is conserved in many animals (fly, zebrafish, mouse and human) and this E164K mutation recapitulated BNCH mutant phenotype. In conclusion, BNCH harboring E164K (BNCH*) was successfully produced by site-directed mutagenesis and cloned into pcDNA.3.1 vector. The construct was transformed into E. coli OmniMAX and that provides a valuable cell assay to search for the molecular ligand of BNCH.
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Cloning of AA9 Polysaccharide Monooxygenase gene AN3860 into pEX2B for expression in Aspergillus oryzae / Nhung Ngo Thi Cam, Van Vu Van // Tạp chí Khoa học và Công nghệ - Đại học Nguyễn Tất Thành . - 2020. - p. 5-10. - ISSN: 2615-9015

Ho Chi Minh City : Nguyen Tat Thanh University, 2020
6 p.
Ký hiệu phân loại (DDC): 660
Polysaccharide monooxygenases (PMOs) catalyze oxidative degradation of recalcitrant carbohydrate chains in cellulose, starch, and chitin. In biofuel industry, the conversion of rich lignocellulose source to fermentable sugars by hydrolytic cellulases can be synergistically boosted by cellulose-active PMOs (AA9 PMOs) found in a vast number of fungi that grow on biomass. Aspergillus nidulans, a filamentous fungus, possess a dozen of PMO-encoding genes, but only the AN3860 is expressed at a high level when cultured with wheat straw as the sole carbon source. Bioinformatic analysis indicates that AN3860 belongs to type 3 AA9 PMO subfamily that is capable of hydroxylating both C1 and C4 of the glycosidic linkages. Therefore, AN3860 may be a potential enzyme to improve cellulose hydrolysis efficiency, which has not been characterized. In this study, we describe the AN3860 cloning into Aspergillus oryzae AUT1-PlD. To facilitate the purification of AN3860, we added a CBM20 tag to its C-terminal. The recombinant vector was designed and constructed successfully. Simultaneously, we have obtained the clone of A. oryzae carrying the target gene by the ATMT method. Further expression optimization and characterization of AN3860 by both activity assays and spectroscopic techniques are underway.
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Multiplicity yours : cloning, stem cell research, and regenerative medicine /Hwa A. Lim
New Jersey : World Scientific, 2006
xxvii, 412 pages. : illustrations (some color) ; 24 cm.
Ký hiệu phân loại (DDC): 176
This is the first book of its kind that treats reproduction, cloning, stem cell research and regenerative medicine in an integrative manner. Touching on the science, social aspects, legal and ethical issues, and the current status of cloning, stem cell research and regenerative medicine, this self-contained book is an excellent source for introducing newcomers to the field or broadening the perspectives of experts and practitioners. In contrast to existing books on the market, which treat each topic in isolation or sensationalize the areas, this book takes an integrative and balanced approach.
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