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Optimization of culture conditions to express AA9 Polysaccharide monooxygenases AN3860 in Escherichia coli / Ngo Thi Cam Nhung, Le Quynh Loan, Vu Van Van // Tạp chí Khoa học và Công nghệ - Đại học Nguyễn Tất Thành . - 2022. - tr. 36-43. - ISSN: 2615-9015
Ký hiệu phân loại (DDC): 615
Lignocellulose biomass is a copious source for second generation biomaterial production. The participant of Polysaccharide monooxygenases enzyme (PMO) in the reactions which convert lignocellulose biomass into monosaccharides enhances the activity and improve the efficiency of hydrolysis of hydrolase enzymes on lignocellulose substrate. Enzyme AN3860, obtained from Aspergillus nidulans strain belonging to AA9 PMO, is expected to catalyze flexibly at C1 and C4 carbon positions of β-glycosidic linkage. As an enzyme with high potential of improving cellulose crystals hydrolysis capacity, AN3860 was successfully cloned into the expression system of E. coli BL21 (DE3) strain. In this study, the culture process of recombinant strain with AN3680 gene is optimized to increase the target proteins yield, thus ensure the outcome of purification process, and save production cost. The results demonstrate that the E. coli recombinant strains grow sufficiently in TB (Terrific Broth) culture media and the highest yield of AN3680 protein achieved when the concentration of Isopropyl β-D-1- thiogalactopyranoside (IPTG) is 0.05 mM and the temperature of the reaction is 30 0C at 150 rpm. After 6 hours of induction, the biomass reaches 500 mg/L and the yield of AN3860 account for (7-10) % total protein generated. The recombinant AN3860 protein is later harvested on larger scale and purified by Ni-NTA column chromatography method for analysis of bioactivities on lignocellulose substrates in the future.
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Optimization of the synthesis reaction and establishing the reference standard of Fenofibrate impurity C (USP) / Tran Van-Hoai Le, Tuyen Ngoc Truong, Thuong Thi-Thuong Le, Dung Thanh Phan // MedPharmRes . - 2025. - tr. 49-61. - ISSN: 2615-9139
Ký hiệu phân loại (DDC): 615.19
Introduction: 1-methylethyl 2-[[2-[4-(4-chlorobenzoyl)phenoxy]-2-methyl propanoyl]oxy]-2-methylpropanoate (or feno- fibrate impurity C, United States Pharmacopoeia [USP]) is an impurity in fenofibrate raw material. To date, publications on synthesis of fenofibrate impurity C (ImpC) have reported low yield and purity, so far. The purpose of this study is to optimize the conditions for synthesis of ImpC using Central Composite Design (CCD) approach and standardization of ImpC as reference substance. Methods: ImpC was synthesized from the reaction between fenofibric acid and isopropyl 2-bromo-2-methylpropanoate in dimethyl sulfoxide in the presence of potassium carbonate. Four factors were investigated: temperature, reaction time, mole ratio of potassium carbonate and isopropyl 2-bromo-2-methylpropanoate. A CCD model was constructed to predict and provide optimal conditions for the reaction. The structure of synthesized ImpC was determined by infrared spectroscopy, mass spectrometry and nuclear magnetic resonance spectroscopy. The procedure for establishing refer- ence standards for ImpC followed the guidelines of ISO 13528:2022. Results: The third-order regression model was developed to predict the yield of reaction using Design Expert software. The optimal conditions were determined: reaction temperature 87 ℃; reaction time 3.64 hours; mole ratio of fenofibric acid and potassium carbonate was 1:4.30; mole ratio of fenofibric acid and isopropyl 2-bromo-2-methylpropanoate was 1:5.95. Under theses conditions, the yield was 89.53%. The structure of ImpC was confirmed. The reference standard of ImpC was established with the assigned value of 99.483%. Conclusions: ImpC was successfully synthesized and standardized as reference standard. The optimal condition for its synthesis was also determined.
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Synthesis of Hydroxychavicol from Eugenol : Graduations Thesis / Tran Thanh Ngoc; Phan Minh Hoang (supervisor)
Ho Chi Minh city : Nguyen Tat Thanh University, 2023
35 p. : image; table ; 29 cm.
Ký hiệu phân loại (DDC): 615.19
Hydroxychavicol has many biological activities such as anti-inflammatory, antimicrobial, antifungal, and antioxidant. Moreover, hydroxychavicol also has anti-platelet aggregation, xanthine oxidase inhibition, and anticancer. The variety of biological activities highlights the high potential of hydroxychavicol as a medicine. The success of synthesizing hydroxychavicol from eugenol on a laboratory scale will be the first step in synthesizing hydroxychavicol on a large scale and commercializing hydroxychavicol. Besides, the synthesized hydroxychavicol
will be a material to semi-synthesize hydroxychavicol derivatives and study their biological activities. Hydroxychavicol was synthesized from eugenol with different catalysts to remove the methyl group from eugenol. The best catalyst was selected and the synthesis procedure was optimized. Thin layer chromatography (TLC) and synthesizing yield were used to evaluate results and choose conditions for the synthesis of hydroxychavicol from eugenol. Column chromatography was used to purify the product and the purity of the product was tested via TLC. Then, the evaluation results of sensory, solubility, and purity tests by the TLC method were used to identify the physical properties of the product. The structure of the compound was determined by mass spectrum (MS) and nuclear magnetic resonance (NMR) spectroscopy. The content of hydroxychavicol in the product was determined by gas chromatography-mass spectrometry (GC-MS).
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